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A Novel Ex Ovo Banding Technique to Alter Intracardiac Hemodynamics in an Embryonic Chicken System
Published on: May 13, 2016
Altered hemodynamics in chick embryos after extraembryonic venous obstruction
M L Broekhuizen1, B Hogers, M C DeRuiter
1Department of Obstetrics and Gynecology, Academic Hospital Rotterdam, Erasmus University, The Netherlands.
Summary
A chick model with venous clip obstruction demonstrated altered cardiac development and hemodynamics. These changes suggest a compensatory mechanism is active during abnormal cardiac development in embryos.
Area of Science:
- Developmental Biology
- Cardiovascular Physiology
- Embryology
Background:
- Abnormal cardiac development, including conotruncal anomalies, poses significant challenges in understanding underlying hemodynamic alterations.
- A novel chick embryo model utilizing extraembryonic venous obstruction (venous clip) was developed to study these phenomena.
Purpose of the Study:
- To investigate hemodynamic changes associated with induced conotruncal anomalies and related malformations in a chick embryo model.
- To correlate hemodynamic data with morphological findings in embryos subjected to venous clip obstruction.
Main Methods:
- Simultaneous measurement of dorsal flow velocities and blood pressures in control and venous clip-applied chick embryos at stages 24 and 34.
- Morphological examination of all embryos post-hemodynamic recordings.
- Statistical analysis to compare hemodynamic and morphological data between control and experimental groups.
Main Results:
- Stage 24 embryos showed impaired looping with decreased peak acceleration.
- Stage 34 embryos exhibited conotruncal malformations, including ventricular septal defects and abnormal valves/arteries.
- Hemodynamically, increased velocities, blood flows, and stroke volume, with reduced heart rate, were observed in venous clip embryos; blood pressures showed no significant difference.
Conclusions:
- The observed hemodynamic alterations in venous clip embryos indicate the activation of a compensatory mechanism.
- This model provides valuable insights into the physiological responses during abnormal cardiac development.
