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Related Experiment Videos

Early developmental changes in intracellular Ca2+ stores in rat brain.

A K Singh1

  • 1Department of Veterinary Diagnostic Medicine, College of Veterinary Medicine, University of Minnesota, St Paul 55108, USA.

Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
|July 30, 1999
PubMed
Summary

Brain intracellular calcium stores mature significantly after birth. The newborn rat brain shows a developed IP3 pathway but an underdeveloped cADPR pathway, while older rats have both, indicating a developmental switch in Ca2+-ATPase function.

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Area of Science:

  • Neuroscience
  • Cell Biology
  • Developmental Biology

Background:

  • Intracellular calcium (Ca2+) signaling is crucial for neuronal function.
  • Developmental changes in Ca2+ handling mechanisms are essential for brain maturation.
  • Understanding these changes is key to comprehending neurological development and disorders.

Purpose of the Study:

  • To investigate the developmental changes in intracellular Ca2+ stores in the rat brain.
  • To compare the functionality of IP3 and cADPR pathways in newborn versus 3-week-old rats.
  • To analyze the developmental expression and regulation of Ca2+-ATPase in the brain.

Main Methods:

  • Measurement of IP3- and cADPR-induced Ca2+ release from synaptosomes.
  • Assay of Ca2+-ATPase activity and 45Ca2+ uptake in ER microsomes.

Related Experiment Videos

  • Assessment of thapsigargin (TG)-induced inhibition of Ca2+-ATPase.
  • Analysis of Ca2+-ATPase gene expression in rat brain neurons.

    • Main Results:

    • Newborn rats showed a less developed cADPR pathway compared to a well-developed IP3 pathway, while 3-week-old rats had robust signaling through both pathways.
    • [3H]IP3, [32P]cADPR, and [3H]Ry binding were significantly lower in newborn samples.
    • Newborn rat brain microsomes exhibited both TG-sensitive and TG-insensitive Ca2+-ATPase forms, whereas older rats predominantly showed the TG-sensitive form, suggesting developmental regulation of the Ca2+-ATPase C-terminal tail.

    Conclusions:

    • The newborn rat brain possesses a functional IP3 pathway but an immature cADPR pathway for intracellular Ca2+ release.
    • A significant developmental transition occurs in Ca2+-ATPase function and regulation as the brain matures.
    • These findings highlight critical developmental shifts in calcium signaling pathways essential for brain development.