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Related Experiment Videos

Engineering a mouse balancer chromosome.

B Zheng1, M Sage, W W Cai

  • 1Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030, USA.

Nature Genetics
|August 4, 1999
PubMed
Summary
This summary is machine-generated.

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Researchers engineered a mouse balancer chromosome using the Cre-loxP system. This genetic tool, featuring a lethal inversion on chromosome 11, enables the maintenance of lethal mutations for genetic screens and research.

Area of Science:

  • Genetics
  • Molecular Biology
  • Mammalian Genetics

Background:

  • Balancer chromosomes are essential genetic tools in Drosophila for stock maintenance and mutagenesis screens.
  • Their application in mice is limited due to difficulties in conventional generation.
  • A need exists for robust balancer systems in mice to facilitate genetic studies.

Purpose of the Study:

  • To engineer a functional mouse balancer chromosome using the Cre-loxP recombination system.
  • To demonstrate its utility in maintaining lethal mutations and facilitating genetic analysis.
  • To establish a strategy for generating similar genetic reagents across the mouse genome.

Main Methods:

  • Engineering a mouse balancer chromosome on chromosome 11 utilizing the Cre-loxP system.

Related Experiment Videos

  • Introducing a 24-centimorgan (cM) inversion between Trp53 and Wnt3, which is recessive lethal.
  • Dominantly marking the balancer with a K14-Agouti transgene.

    • Main Results:

    • The engineered inversion suppresses crossing over within the inverted region while increasing recombination in flanking areas.
    • The balancer chromosome successfully maintained a recessive lethal mutation in a trans-heterozygous stock.
    • The developed strategy is adaptable for creating additional balancer chromosomes throughout the mouse genome.

    Conclusions:

    • The Cre-loxP system provides an effective method for engineering mouse balancer chromosomes.
    • This engineered balancer facilitates the study of lethal mutations and supports large-scale mutagenesis programs in mice.
    • This advancement is crucial for comprehensive functional analyses of the mouse genome.