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Identification of Homologous Recombination Events in Mouse Embryonic Stem Cells Using Southern Blotting and Polymerase Chain Reaction
Published on: November 20, 2018
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Genome Wide Conditional Mouse Knockout Resources
C Kaloff1, K Anastassiadis2, A Ayadi3
1Institute of Developmental Genetics, Helmholtz Zentrum Muenchen, D-85764 Neuherberg, Germany.
Drug Discovery Today. Disease Models
|August 12, 2024
Summary
The International Knockout Mouse Consortium created over 18,500 gene mutations in mouse embryonic stem cells, resulting in thousands of mutant mouse strains and cre-driver lines for research. These resources are available at www.mousephenotype.org.
Area of Science:
- * Genetics and Genomics
- * Mammalian Genetics
- * Mouse Models
Background:
- * The International Knockout Mouse Consortium (IKMC) established high-throughput pipelines for gene trapping and targeting.
- * This initiative generated conditional mutations for over 18,500 genes in C57BL/6N mouse embryonic stem (ES) cells.
- * These ES cell lines are archived and available as a frozen resource for the research community.
Purpose of the Study:
- * To create and provide access to a comprehensive library of mouse models for genetic research.
- * To facilitate the analysis of gene-disease associations through standardized mouse strains.
- * To support biomedical research by offering a diverse collection of genetic tools.
Main Methods:
- * High-throughput gene trapping and gene targeting in mouse ES cells.
- * Production of conditional mutations for a large number of genes.
- * Development of inducible cre-driver mouse strains and cre-driver rAAVs.
Main Results:
- * Over 18,500 genes have conditional mutations in archived ES cells.
- * More than 6,000 mutant mouse strains have been generated by IKMC and the International Mouse Phenotyping Consortium (IMPC).
- * A resource of 250 inducible cre-driver mouse strains and 27 cre-driver rAAVs was established.
Conclusions:
- * The IKMC/IMPC resource provides a standardized library of mouse models with defined genetic backgrounds.
- * These resources enable the analysis of gene-disease associations in diverse genetic contexts.
- * The IKMC/IMPC resource is expected to significantly impact biomedical research.
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