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Reasons and limits of substrate activity of modified L-dNTP in DNA biosynthesis
A A Krayevsky1, N B Dyatkina, D G Semizarov
1Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Moscow, Russia.
Nucleosides & Nucleotides
|August 5, 1999
Abstract:
Theoretical and experimental analysis of interaction of modified D- and L- dNTP as substrates for template-dependent and template-independent DNA polymerases was performed. It is shown that if the modified nucleoside 5'-triphosphates do not contain a substituent in position 3' DNA chains can be extended by both strereoisomeric series with the same kinetic parameters. But the presence of even a 3'-hydroxy group in L-dNTP prevents their incorporation into the DNA chain.