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Quantification of lamivudine (3TC) by competitive immunoassay
F Akeb1, C Creminon, M C Nevers
1Laboratoire de Chimie Bio-Organique, ESA CNRS 6001, Faculté des Sciences, Université de Nice-Sophia Antipolis, Nice, France.
Nucleosides & Nucleotides
|August 5, 1999
Abstract:
Lamivudine or 3TC, the (-) eniantiomer of 2'-deoxy-3'-thiacytidine, is a prototype of a novel class of levogyre dideoxynucleosides analogues used in treatment of HIV and HBV infection. We describe a method corresponding to the first enzyme immunoassay for quantifying this antiviral drug. This technique use an enzyme conjugate that not require the use of radioactive labelling. In this study, anti-3TC antibodies were raised in rabbits by immunising with 3TC-HS-kelhoyle limpet hemocyanin (KLH) conjugate.