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Development of a new DNA sequencing method: 3'-ester cleavage catalyzed by Taq DNA polymerase
1Unité de Chimie Organique, Institut Pasteur, Paris, France.
Nucleosides & Nucleotides
|August 5, 1999
Abstract:
A new 3'-esterified dTTP is incorporated into DNA by Taq DNA polymerase but does not act as a chain terminator. The esterase activity of the polymerase seems to be template dependent and occurs only if the next correct nucleotide is present.