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Related Experiment Videos

Distribution and function of the adhesion molecule BEN during rat development.

J P Stephan1, L Bald, P E Roberts

  • 1Department of Protein Chemistry, Genentech, Inc., South San Francisco, California, 94080-4990, USA.

Developmental Biology
|August 6, 1999
PubMed
Summary

The BEN protein (Ag 2117), initially found in the notochord, regulates pancreatic epithelial cell growth and PDX1 expression, crucial for insulin gene development in rats.

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Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Cell Biology

Background:

  • The notochord significantly influences adjacent neural and mesodermal tissue development.
  • The notochord's role in dorsal pancreas differentiation is known, but signaling pathways remain unclear.
  • Identifying novel proteins is key to understanding pancreatic development.

Purpose of the Study:

  • To identify proteins involved in embryonic pancreatic differentiation.
  • To characterize the function of the cloned cell surface antigen 2117 (Ag 2117), the rat homologue of BEN.
  • To investigate the role of BEN in pancreatic development.

Main Methods:

  • Monoclonal antibodies were raised against embryonic pancreatic ductal epithelial cell lines (BUD and RED).
  • The cell surface antigen 2117 (Ag 2117) was cloned using MAb 2117.

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  • BEN expression patterns and its effects on pancreatic cells were analyzed in rat models and organ cultures.

    • Main Results:

    • Ag 2117, the rat homologue of BEN, is initially expressed exclusively in the notochord during early rat development.
    • Ag 2117 regulates pancreatic epithelial cell growth via the ras and Jun kinase pathways.
    • Ag 2117 influences the expression of PDX1, a transcription factor vital for insulin gene expression.

    Conclusions:

    • BEN (Ag 2117) plays a significant role in rat pancreatic development.
    • BEN signaling impacts pancreatic epithelial cell proliferation and differentiation.
    • This study identifies BEN as a key regulator in the pancreatic development pathway.