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Related Experiment Videos

LDH elevation after autologous stem cell transplantation.

B Bolwell1, B Pohlman, M Kalaycio

  • 1Department of Hematology and Medical Oncology, and Transplant Center, Cleveland Clinic Foundation, OH, USA.

Bone Marrow Transplantation
|August 6, 1999
PubMed
Summary

Serum lactate dehydrogenase (LDH) levels universally rise after autologous peripheral blood progenitor cell (PBPC) infusion, peaking on day +1. This increase is linked to ex vivo cell lysis during PBPC collection, not in vivo lysis.

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Area of Science:

  • Hematology
  • Transplant Medicine
  • Clinical Biochemistry

Background:

  • Serum lactate dehydrogenase (LDH) elevation is anecdotally observed post-autologous peripheral blood progenitor cell (PBPC) infusion.
  • The presumed cause is cellular lysis, but the exact mechanism and correlation require further investigation.

Purpose of the Study:

  • To investigate the incidence and pattern of serum LDH changes following autologous PBPC transplantation.
  • To identify factors correlating with post-infusion LDH elevation.

Main Methods:

  • Retrospective review of 203 patients undergoing autologous PBPC transplant.
  • Analysis of serum LDH values from day -1 to day +5 post-transplant.
  • Correlation analysis between LDH levels, CD34+ cell dose, and number of apheresis procedures.

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Main Results:

  • 99% of patients showed a rise in serum LDH post-PBPC infusion, with 92% peaking on day +1.
  • 76% of patients with initially normal LDH experienced elevation; 22% with high LDH saw further increases.
  • LDH increase correlated with the number of apheresis procedures, not directly with the infused CD34+ cell dose.

Conclusions:

  • Serum LDH universally increases after autologous PBPC infusion, peaking on day +1.
  • The rise is attributed to ex vivo cell lysis during apheresis, potentially due to increased red blood cell contamination and hemolysis.
  • The number of apheresis procedures is a key factor associated with post-transplant LDH elevation.