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Comparison of three sperm preparation media
R K Sharma1, K Seifarth, D Garlak
1Department of Urology, The Cleveland Clinic Foundation, Ohio 44195, USA.
International Journal of Fertility and Women'S Medicine
|August 6, 1999
Summary
SpermFertil medium resulted in lower sperm quality compared to ISolate and Perwash. ISolate is a viable alternative for sperm separation in assisted reproduction following Percoll withdrawal.
Area of Science:
- Reproductive Medicine
- Sperm Preparation Techniques
- Assisted Reproductive Technology
Background:
- Sperm separation from seminal plasma is crucial for capacitation and fertilization in assisted reproduction.
- Percoll, a common sperm separation medium, was withdrawn from the market due to safety concerns.
- Alternative sperm washing media are needed to maintain sperm quality for assisted reproduction procedures.
Purpose of the Study:
- To evaluate and compare the sperm quality after separation using two novel sperm washing media: ISolate and SpermFertil.
- To compare the outcomes with a Percoll-type medium (Perwash) as a benchmark.
Main Methods:
- Semen samples from 10 normozoospermic men were processed using Perwash, ISolate, and SpermFertil.
- Key semen parameters assessed included sperm count, motility, velocity, viability, and morphology (WHO and Kruger criteria).
- Sperm motility was monitored over three hours to assess sample longevity.
Main Results:
- SpermFertil yielded significantly lower sperm count, motility, velocity, and normal morphology compared to ISolate and Perwash (P < .05).
- Tail abnormalities were more frequent with SpermFertil (P < .001).
- ISolate and Perwash demonstrated comparable sperm quality and motility duration, superior to SpermFertil.
Conclusions:
- SpermFertil significantly compromises sperm quality compared to ISolate and Perwash.
- ISolate provides comparable sperm quality to Percoll-type media, making it a suitable alternative for sperm preparation in assisted reproduction.