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Quercetin down-regulated bcl-2 gene expression in human leukemia HL-60 cells
Zhongguo Yao Li Xue Bao = Acta Pharmacologica Sinica
|August 7, 1999
Abstract
Aim:
To study the effect of quercetin (Que) on bcl-2 gene expression in human leukemia HL-60 cells.
Methods:
Immunohistochemical analysis and RNA Dot blot hybridization were used to identify the expression of bcl-2 genes.
Results:
The expression of bcl-2 protein was 94% in control HL-60 cells, which became 45%-84% when they were cultured with Que 15-60 mumol.L-1 for 48 h. The expression of bcl-2 mRNA in HL-60 cells was obviously decreased in treatment with Que 15-60 mumol.L-1.
Conclusion:
The apoptotic action of Que in HL-60 cells was associated with the down-regulation of the apoptosis-suppressing gene bcl-2.