Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multimerin processing by cells with and without pathways for regulated protein secretion.

C P Hayward1, Z Song, S Zheng

  • 1Departments of Pathology and Molecular Medicine, Laboratory Medicine, and Medicine, McMaster University, Hamilton, Ontario, Canada.

Blood
|August 10, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From Thomas Mann to John Green, how authors and books shape our understanding of TB.

IJTLD open·2026
Same author

Robotic-assisted anterior and posterior cervical spine surgeries.

Musculoskeletal surgery·2025
Same author

Redirecting venous flow from the superior mesenteric vein to the inferior mesenteric vein in resections for locally advanced pancreatic cancer.

Langenbeck's archives of surgery·2025
Same author

Fighting tuberculosis hand in hand: A call to engage communities affected by TB as essential partners in research.

PLOS global public health·2025
Same author

TB case fatality and recurrence in a private sector cohort in Mumbai, India.

The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2021
Same author

Few-fs resolution of a photoactive protein traversing a conical intersection.

Nature·2021
Same journal

Phosphorylated DEK sustains leukemia stem cells by enabling PBX3-driven transcriptional reprogramming.

Blood·2026
Same journal

A single-cell atlas identifies oncogenic transcriptional programs and immune escape mechanisms in CTCL.

Blood·2026
Same journal

TRANSFORM-1 Phase 3 study: Efficacy and safety of navitoclax plus ruxolitinib in patients with untreated myelofibrosis.

Blood·2026
Same journal

Antiphospholipid syndrome (APS) is a platelet factor 4 (PF4)-centric immunothrombotic disorder.

Blood·2026
Same journal

CD4+CD25+FOXP3+ Regulatory T Cells to Protect Against Graft Versus Host Disease.

Blood·2026
Same journal

Arterial iron regulates vasodilation during anemia via endothelial holo α-globin.

Blood·2026
See all related articles

Multimerin, a factor V-binding protein, is processed differently in various cells. Endothelial cells constitutively secrete multimerin, while regulated secretion cells show altered processing, impacting its function.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Protein Science

Background:

  • Multimerin is a large, soluble protein found in platelet alpha-granules and vascular endothelium.
  • Endothelial cells store multimerin in granules lacking P-selectin and constitutively secrete it.
  • The posttranslational processing and storage of multimerin are not fully understood.

Purpose of the Study:

  • To investigate the posttranslational processing and storage of multimerin in different cellular contexts.
  • To compare multimerin processing in cells with constitutive versus regulated secretion pathways.
  • To identify factors influencing multimerin's final form and secretion.

Main Methods:

  • Expression of human endothelial cell prepromultimerin in various cell lines (e.g., HEK 293, neuroendocrine cells).

Related Experiment Videos

  • Analysis of recombinant multimerin for variations in glycosylation, proteolytic processing, and multimer profile.
  • Comparison of multimerin processing in cells with constitutive and regulated secretion pathways.

    • Main Results:

    • Recombinant multimerin exhibited diverse glycosylation, proteolytic processing, and multimer profiles across different cell lines.
    • Human embryonic kidney 293 cells replicated the constitutive secretion processing seen in endothelial cells.
    • Multimerin targeted for regulated secretion underwent more extensive proteolysis than in platelets.
    • Specific endoproteases, potentially from megakaryocytes, may be necessary for platelet-type multimerin production.

    Conclusions:

    • Cell type and secretion pathway significantly influence multimerin's posttranslational processing and storage.
    • Constitutive secretion in endothelial cells involves specific processing pathways.
    • Regulated secretion pathways lead to altered, more extensive proteolysis of multimerin.
    • Tissue-specific processing differences may impact multimerin's functions and its interaction with factor V.