Instability of chromosome 7 in colony forming cells of patients with aplastic anemia
Related Experiment Video
Updated: Oct 19, 2026

Colony-Forming Unit Assay Adapted For Patient-Derived Bone Marrow and Peripheral Blood Mononuclear Cells
Published on: June 26, 2026
1Department of Pediatrics, Hiroshima University School of Medicine, Japan.
Abstract:
Monosomy 7 in isolated bone marrow mononuclear cells and colony forming cells from patients with aplastic anemia who later developed myelodysplastic syndrome/leukemia with monosomy 7 were examined by fluorescence in situ hybridization. Colonies derived from bone marrow mononuclear cells of the aplastic anemia patients consisted of a mixture of cells of normal karyotype and monosomy 7, ranging from 0 to 97.2%. This result suggests that colony forming cells initially had a normal karyotype but then lost chromosome 7 during growth in the semisolid culture. This finding suggests the genetically unstable condition of chromosome 7 in colony forming cells in aplastic anemia patients. This chromosome instability of colony forming cells may lead to malignant transformation.
Related Concept Videos
Separation of Sister Chromatids
At the onset of anaphase, separase, a proteolytic enzyme, is...
Microtubule Instability
Karyotyping
Abnormal Proliferation
DNA Damage can Stall the Cell Cycle
Anaphase Promoting Complex

