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Urocortin: slower dissociation than corticotropin releasing factor from the CRF binding protein
S Henriot1, F M Dautzenberg, G J Kilpatrick
1Pharma Division, F. Hoffmann-La Roche, Basel, Switzerland.
Abstract:
We report on a comparison of the kinetics of [125I][Tyr0]corticotropin releasing factor (CRF) and [125I][Tyr0]urocortin binding to the CRF binding protein (CRF-BP) at physiological temperature. The association rates of [125I][Tyr0]urocortin or [125I][Tyr0]CRF binding to the CRF-BP were similar. The half time of association for [125I][Tyr0]urocortin was 3.2 min and for [125I][Tyr0]CRF, 2.6 min. [125I][Tyr0]urocortin dissociated from the CRF-BP but the rate of dissociation was slower than for [125I][Tyr0]CRF. The half time for dissociation of [125I][Tyr0]urocortin was 131 min and for [125I][Tyr0]CRF, 64 min. This slower dissociation indicates that the CRF-BP may be more effective in clearing urocortin than CRF.
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