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Related Experiment Videos

PCR-Based methods for genotyping viridans group streptococci.

S Alam1, S R Brailsford, R A Whiley

  • 1Joint Microbiology Research Unit, Guy's, King's and St. Thomas' Dental Institute, London SE5 9RW, England.

Journal of Clinical Microbiology
|August 17, 1999
PubMed
Summary
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Repetitive element PCR methods effectively distinguish strains of viridans group streptococci. These simple PCR techniques are reproducible and suitable for clinical and ecological studies of these bacteria.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Bacterial Genetics

Background:

  • Viridans group streptococci comprise numerous species, making accurate strain typing crucial for clinical and ecological studies.
  • Traditional methods for bacterial strain differentiation can be labor-intensive and time-consuming.

Purpose of the Study:

  • To evaluate the efficacy of three repetitive element PCR-based methods for typing human viridans group streptococci.
  • To assess the reproducibility and discriminatory power of these PCR methods.

Main Methods:

  • DNA was extracted from 72 unique isolates of 15 viridans group streptococci species by boiling.
  • Standard repetitive extragenic palindromic (REP)-PCR, enterobacterial repetitive intergenic consensus-PCR, and Salmonella enteritidis repetitive element-PCR were employed for bacterial strain typing.

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Main Results:

  • All three PCR-based typing methods demonstrated high reproducibility.
  • The methods successfully distinguished between the 72 unique isolates, with no two isolates showing greater than 90% similarity.
  • Isolates identified as identical by REP-PCR were also identical by the other two methods.

Conclusions:

  • Repetitive element PCR methods are simple, reproducible, and effective for differentiating strains within the viridans group streptococci.
  • While not suitable for species identification, these PCR techniques are valuable tools for clinical and ecological investigations of viridans group streptococci.