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Discrimination of multidrug-resistant Mycobacterium tuberculosis IS6110 fingerprint subclusters by rpoB gene mutation
I Portugal1, S Maia, J Moniz-Pereira
1Unidade dos Retrovírus e Infecções Associadas and Centro de Patogénese Molecular, Faculdade de Farmácia da Universidade de Lisboa, 1649-019 Lisbon, Portugal. isabel.portugal@ff.ul.pt
Journal of Clinical Microbiology
|August 17, 1999
Abstract:
The rpoB gene mutations in a 69-bp region of the gene, resulting in resistance to rifampin, were used to discriminate between Mycobacterium tuberculosis IS6110 fingerprint subclusters. These subclusters exhibited identical IS6110 fragments or had one or two additional fragments. In the two major subclusters all the analyzed strains have the same variant rpoB allele but are different from each other, suggesting the occurrence of independent outbreaks.