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Related Experiment Videos

Systematic changes in gene expression patterns following adaptive evolution in yeast.

T L Ferea1, D Botstein, P O Brown

  • 1Department of Genetics, Stanford University School of Medicine, Stanford, CA 94305-5120, USA.

Proceedings of the National Academy of Sciences of the United States of America
|August 18, 1999
PubMed
Summary
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Adaptive evolution in Saccharomyces cerevisiae selects for fitter strains. Gene expression analysis revealed altered central metabolism, favoring complete glucose oxidation over fermentation for increased fitness.

Area of Science:

  • Evolutionary biology
  • Microbial genetics
  • Systems biology

Background:

  • Adaptive evolution studies model natural selection.
  • Saccharomyces cerevisiae is a model organism for evolutionary studies.
  • Continuous culture selects for genetic variants conferring higher fitness.

Purpose of the Study:

  • To investigate adaptive evolution in Saccharomyces cerevisiae under glucose limitation.
  • To identify genetic and regulatory changes associated with increased fitness.
  • To compare genome-wide gene expression patterns between evolved and ancestral strains.

Main Methods:

  • Culturing Saccharomyces cerevisiae in glucose-limited chemostats for over 250 generations.
  • Generating independently evolved strains from a clonal population.

Related Experiment Videos

  • Utilizing DNA microarrays for genome-wide gene expression profiling.

    • Main Results:

    • Several hundred genes exhibited significantly altered expression in evolved strains compared to the parental strain.
    • Consistent alterations in gene expression were observed across three independent evolved lineages.
    • Affected genes were primarily involved in central carbon metabolism, including glycolysis, the tricarboxylic acid cycle, and oxidative phosphorylation.

    Conclusions:

    • Increased fitness in evolved Saccharomyces cerevisiae is linked to altered regulation of central metabolism.
    • Evolved strains exhibit a metabolic shift towards complete glucose oxidation rather than fermentation.
    • This metabolic adaptation optimizes resource utilization and enhances growth under selective pressure.