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Identification and localisation of ATP P2X receptors in rat midbrain
R A Worthington1, T V Arumugam, M A Hansen
1Institute for Biomedical Research, and Department of Anatomy and Histology, The University of Sydney, NSW, Australia.
Abstract:
P2X receptors that are gated by extracellular ATP are among the few known examples of ligand-gated cation-selective channels. There have been seven cloned proteins identified to date as members of the P2X receptor family in a wide range of tissues from the peripheral and central nervous systems and from many species. To determine the distribution of the P2X subtypes in the rat midbrain, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE)/Western blotting was combined with immunolocalisation using confocal microscopy. Subtypes P2X1-6 were detected in the periaqueductal gray area and the ependymal layer bordering the ventricle with a widespread distribution.