Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Oligonucleotide-peptide conjugates as potential antisense agents.

E M Zubin1, E A Romanova, E M Volkov

  • 1Chemical Department and A.N. Belozersky Institute of Physical Chemical Biology, M.V. Lomonosov Moscow State University, Russia.

FEBS Letters
|August 19, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HspBP1 in Complex with the Peptide of the Innate Immunity Protein Tag7 is Able to Lyse Tumor Cells Carrying TNFR1 Receptor.

Doklady. Biochemistry and biophysics·2024
Same author

[Isolated splenic lymphangioma].

Khirurgiia·2023
Same author

Incubation of Lymphocytes with IL-2 Causes the Appearance of Antitumor T Cells Carrying FC, CD25, and LFA-1 on the Surface.

Doklady. Biochemistry and biophysics·2023
Same author

Tag7-Mts1 Complex Activates Chemotaxis of Regulatory T Cells.

Doklady. Biochemistry and biophysics·2022
Same author

Modified Oligonucleotides: New Structures, New Properties, and New Spheres of Application.

Russian journal of bioorganic chemistry·2021
Same author

[MutL Protein from the Neisseria gonorrhoeae Mismatch Repair System: Interaction with ATP and DNA].

Molekuliarnaia biologiia·2021

Researchers developed a new method for creating oligonucleotide-peptide conjugates. This technique improves antisense agents by linking peptides to oligonucleotides for better RNA interference within cells.

Area of Science:

  • Bioconjugation Chemistry
  • Molecular Biology
  • Drug Discovery

Background:

  • Oligonucleotide-peptide conjugates show promise for therapeutic applications, particularly as antisense agents.
  • Existing conjugation methods may lack robustness or broad applicability for creating these complex molecules.

Purpose of the Study:

  • To develop a novel and generally applicable conjugation chemistry for creating oligonucleotide-peptide conjugates.
  • To facilitate the use of oligonucleotide-peptide conjugates as improved antisense agents for RNA interference.

Main Methods:

  • A fragment coupling approach was employed, utilizing pre-synthesized peptides.
  • Conjugation was achieved at the 2'-position of a selected nucleotide.
  • The oligonucleotide chain was maintained in a protected state and attached to a solid support during conjugation.

Related Experiment Videos

Main Results:

  • A robust method for synthesizing oligonucleotide-peptide conjugates was successfully developed.
  • The novel chemistry allows for the specific attachment of peptides to oligonucleotides.
  • The approach is designed for general applicability in creating diverse conjugates.

Conclusions:

  • The developed fragment coupling strategy provides a versatile platform for oligonucleotide-peptide conjugate synthesis.
  • This method enhances the potential for creating improved antisense agents for therapeutic applications.
  • The robust and generally applicable nature of the chemistry supports further development in RNA interference strategies.