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Antioxidant activity: what do we measure?

G Bartosz1, M Bartosz

  • 1Department of Molecular Biophysics, University of Lódź, Poland. gbartosz@biol.uni.lodz.pl

Acta Biochimica Polonica
|August 24, 1999
PubMed
Summary
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Antioxidants primarily work by reducing oxidized compounds, not by preventing their formation. This study reveals their main action is reducing the ABTS+* cation radical, impacting antioxidant assays.

Area of Science:

  • Biochemistry
  • Chemical Kinetics

Background:

  • Antioxidant activity is often measured by the inhibition of oxidation.
  • The precise mechanisms by which antioxidants function in these assays require further elucidation.

Purpose of the Study:

  • To investigate the mechanism of antioxidant action in the 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assay.
  • To determine whether antioxidants inhibit radical formation or reduce oxidized products.

Main Methods:

  • Studied the inhibition of ABTS oxidation by free radicals generated from 2,2'-azobis(2-amidopropane) (ABAP).
  • Correlated antioxidant ability to delay ABTS oxidation with reduction kinetics of the ABTS+* cation radical.
  • Examined the effect of antioxidant addition timing on peroxynitrite-induced ABTS+* formation.

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Main Results:

  • A direct correlation exists between delaying ABTS oxidation and rapid reduction of ABTS+*.
  • A correlation was also found between reducing the maximal rate of ABTS oxidation and slow reduction of ABTS+*.
  • Antioxidant addition timing did not affect the reduction of peroxynitrite-induced ABTS+* formation.

Conclusions:

  • The primary role of antioxidants in this system is the reduction of the ABTS+* cation radical, not the prevention of its formation.
  • Antioxidant assays may predominantly reflect the capacity to reduce oxidation products rather than inhibit radical generation.