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Effect of adenosine derivatives on in vitro thrombus formation induced by shear stress
M Menegatti1, G Cristalli, L Gallo
1Angelo Bianchi Bonomi Haemophilia and Thrombosis Center, Institute of Internal Medicine, IRCCS Maggiore Hospital and University of Milan, Italy.
Background And Objective:
Shear-stress is considered to be the first event of platelet aggregation in vivo and platelet adhesion may be enhanced under pathologic conditions (e.g. arterial occlusion).
Design And Methods:
We wanted to test the effect of adenosine derivatives on platelet aggregation induced by shear-stress. By increasing platelet cAMP adenosine derivatives inhibit platelet activation. This in turn leads to P-selectin (CD62P) exposure, which is known to play a fundamental role in the binding of platelets to leukocytes. This gives rise to thrombus formation.
Results:
The levels of cAMP (pmol/mL) prior to and after treating blood with the following compounds were respectively: PGE1 4.67+/-0.29, 9.33+/-0. 58; SP64 5.2+/-0.34, 6.83+/-0.52; 2-Cl-adenosine 5.83+/- 0.58, 7. 45+/-0.55; NECA 7.00+/-2.29, 8.00+/-1.76.
Interpretation And Conclusions:
High shear rate was studied using a filteraggregometer which could be a good test for analyzing what happens under physiologic conditions compared to other systems in which platelet aggregation only occurs after adding aggregating agents (Born aggregometer).