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Problems in predicting cell damage from bubble bursting.

D Dey1, A N Emery

  • 1School of Chemical Engineering, University of Birmingham, Edgbaston, Birmingham B15 2TT, UK.

Biotechnology and Bioengineering
|August 24, 1999
PubMed
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Bubble jet characteristics do not predict cell damage in animal cell cultures. Protective agents like fetal calf serum (FCS) and Pluronic F-68 significantly reduce cell death from bubble sparging.

Area of Science:

  • Biotechnology
  • Cell Biology
  • Bioprocess Engineering

Background:

  • Cell damage in sparged cultures is a concern for bioprocesses.
  • Bubble bursting is a potential source of mechanical stress.
  • Protective agents are used to mitigate cell damage.

Purpose of the Study:

  • To determine if single bubble burst parameters can predict cell damage.
  • To investigate the protective effects of fetal calf serum (FCS) and Pluronic F-68.
  • To correlate bubble burst characteristics with cell death rates.

Main Methods:

  • High-speed video microscopy to analyze bubble burst profiles.
  • Sparging of TBC3 hybridoma cells in culture.
  • Measurement of bubble jet height and speed.

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  • Quantification of cell death rate constants.

    • Main Results:

    • FCS and Pluronic F-68 reduced cell damage even at low concentrations.
    • Single bubble burst parameters (jet height, speed) did not correlate with cell death rates.
    • Surface properties also showed no usable correlation with death rate constants.

    Conclusions:

    • Bubble burst parameters are not reliable predictors of cell damage in sparged cultures.
    • Protective agents like FCS and Pluronic F-68 are effective in reducing bubble-induced cell death.
    • Further research is needed to understand the mechanisms of cell protection.