Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nitric oxide and intestinal barrier failure.

E P Nadler1, J S Upperman, E C Dickinson

  • 1Department of Surgery, Children's Hospital of Pittsburgh, PA 15213, USA.

Seminars in Pediatric Surgery
|August 26, 1999
PubMed
Summary

Systemic inflammatory response syndrome (SIRS) involves gut barrier injury. Nitric oxide (NO) upregulation causes peroxynitrite formation, leading to enterocyte apoptosis and bacterial translocation, potentially inciting SIRS.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Thirty-day outcomes for children and adolescents undergoing laparoscopic sleeve gastrectomy at a free-standing children's hospital.

Clinical obesity·2017
Same author

Melanocortin-4 receptor signaling is not required for short-term weight loss after sleeve gastrectomy in pediatric patients.

International journal of obesity (2005)·2015
Same author

Health informatics for pediatric disaster preparedness planning.

Applied clinical informatics·2013
Same author

Management of congenital giant inguinal scrotal hernias in the newborn.

Hernia : the journal of hernias and abdominal wall surgery·2011
Same author

NF-kappaB-mediated expression of MAPK phosphatase-1 is an early step in desensitization to TLR ligands in enterocytes.

Mucosal immunology·2010
Same author

Evidence vs experience in the surgical management of necrotizing enterocolitis and focal intestinal perforation.

Journal of perinatology : official journal of the California Perinatal Association·2008

Area of Science:

  • Gastroenterology
  • Immunology
  • Cellular Biology

Background:

  • Systemic inflammatory response syndrome (SIRS) is a major cause of death.
  • Proinflammatory mediators are implicated in SIRS, but their mechanisms are unclear.
  • Nitric oxide (NO) may regulate gut barrier function.

Purpose of the Study:

  • To investigate the role of nitric oxide (NO) in the pathogenesis of SIRS.
  • To elucidate the mechanisms by which NO contributes to intestinal injury and bacterial translocation.

Main Methods:

  • The study likely involved investigating the effects of NO upregulation on intestinal epithelial cells.
  • Analysis of mitochondrial function, caspase activation, and apoptosis was probably performed.
  • Mechanisms of peroxynitrite formation and its downstream effects were examined.

Related Experiment Videos

Main Results:

  • Sustained NO upregulation in the intestine leads to peroxynitrite formation.
  • Peroxynitrite damages mitochondria, inhibits respiration, and triggers apoptosis in enterocytes.
  • Enterocyte apoptosis creates gaps in the gut barrier, allowing bacterial translocation.

Conclusions:

  • NO-induced enterocyte apoptosis contributes to the pathogenesis of SIRS.
  • Disruption of the gut barrier by NO plays a critical role in bacterial translocation.
  • Targeting NO pathways may offer therapeutic strategies for SIRS.