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Updated: Aug 12, 2026
Cholesterol Efflux Assay
Published on: March 6, 2012
Sandwich enzyme-linked immunosorbent assay for plasma cholesteryl ester transfer protein concentration
Objectives:
Cholesteryl ester transfer protein (CETP) mediates the transfer of HDL cholesterol to apoB-containing lipoproteins. Its mass and activity are increased in several pro-atherogenic conditions. The objective of this study is to develop a cost- and time-effective sandwich ELISA for plasma CETP concentration.
Design And Methods:
Monoclonal anti-CETP, TP20, was used as the capture antibody, while the other biotinylated monoclonal anti-CETP, TP2, was used for detection. The results were expressed in an arbitrary unit, ng biotin-TP2 bound per microl plasma. Plasma CETP concentrations, activities and their relationship were assessed in 35 IDDM children.
Results:
The assay had an intra-assay CV of 8.75% and an inter-assay CV under 10%. Plasma CETP concentration of these subjects ranged from 0.36-1.89 ng biotin-TP2/microL. CETP concentration was significantly correlated with CETP activity (r = 0.51, p < 0.01).
Conclusion:
The sandwich ELISA we have developed carried sufficient sensitivity for assaying plasma CETP concentration in human.
