Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Polymer-cushioned bilayers. I. A structural study of various preparation methods using neutron reflectometry.

J Y Wong1, J Majewski, M Seitz

  • 1Department of Chemical Engineering, University of California, Santa Barbara, California 93106, USA.

Biophysical Journal
|August 31, 1999
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prognostic Predictions for Patients with Glioblastoma after Standard Treatment: Application of Contrast Leakage Information from DSC-MRI within Nonenhancing FLAIR High-Signal-Intensity Lesions.

AJNR. American journal of neuroradiology·2019
Same author

A Compressed-Sensing Based Blind Deconvolution Method for Image Deblurring in Dental Cone-Beam Computed Tomography.

Journal of digital imaging·2018
Same author

Eliminating artifacts in single-grid phase-contrast x-ray imaging for improving image quality.

Computers in biology and medicine·2018
Same author

Effect of the sexual abstinence period recommended by the World Health Organization on clinical outcomes of fresh embryo transfer cycles with normal ovarian response after intracytoplasmic sperm injection.

Andrologia·2018
Same author

Type 2 diabetes mellitus and risk of open-angle glaucoma development in Koreans: An 11-year nationwide propensity-score-matched study.

Diabetes & metabolism·2017
Same author

Effect of rapamycin treatment during post-activation and/or in vitro culture on embryonic development after parthenogenesis and in vitro fertilization in pigs.

Reproduction in domestic animals = Zuchthygiene·2017

This study on polymer-cushioned lipid bilayers found that initial polymer layer conditions critically affect bilayer structure. Successful continuous bilayers were formed using vesicle adsorption and Langmuir-Blodgett deposition, excluding horizontal deposition.

Area of Science:

  • Materials Science
  • Biophysics
  • Surface Chemistry

Background:

  • Lipid bilayers are crucial for cell membranes.
  • Creating stable, biomimetic lipid bilayers on surfaces is challenging.
  • Polymer cushions can enhance lipid bilayer stability and mimic cellular environments.

Purpose of the Study:

  • To evaluate different preparation methods for polymer-cushioned lipid bilayers.
  • To determine the optimal conditions for forming continuous dimyristoylphosphatidylcholine (DMPC) bilayers on polyethylenimine (PEI) substrates.
  • To assess the influence of polymer layer preparation on bilayer structure.

Main Methods:

  • Neutron reflectometry was used to analyze bilayer structures.
  • Four distinct methods were employed for depositing DMPC bilayers onto PEI-coated quartz.

Related Experiment Videos

  • Techniques included vesicle adsorption and Langmuir-Blodgett deposition.

    • Main Results:

    • The initial state of the polymer layer is critical for successful bilayer formation.
    • Continuous polymer-supported lipid bilayers were achieved via vesicle adsorption and vertical Langmuir-Blodgett deposition.
    • Horizontal deposition of lipid monolayers resulted in unsuccessful bilayer formation.

    Conclusions:

    • The preparation method significantly impacts the integrity of polymer-cushioned lipid bilayers.
    • These polymer-supported bilayers offer a promising model for studying transmembrane proteins in near-native conditions.
    • Understanding preparation methods is key to developing advanced biomimetic membrane systems.