Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Nucleosome mobilization catalysed by the yeast SWI/SNF complex.

I Whitehouse1, A Flaus, B R Cairns

  • 1Division of Gene Regulation, University of Dundee, UK.

Nature
|August 31, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Amyloid PET imaging in France: One-year experience and perspectives.

Revue neurologique·2025
Same author

FDG PET biomarkers for prediction of survival in metastatic melanoma prior to anti-PD1 immunotherapy.

Scientific reports·2021
Same author

Cigarette smoking significantly alters sperm DNA methylation patterns.

Andrology·2017
Same author

Genome-wide DNA methylation profiling in zebrafish.

Methods in cell biology·2016
Same author

The impact of chromosomal translocation locus and fusion oncogene coding sequence in synovial sarcomagenesis.

Oncogene·2016
Same author

IRS2 integrates insulin/IGF1 signalling with metabolism, neurodegeneration and longevity.

Diabetes, obesity & metabolism·2014
Same journal

Daily briefing: How cooperation built the world.

Nature·2026
Same journal

Deep-sea oddities and boatloads of other new species - June's best science images.

Nature·2026
Same journal

From cloning to gene-editing: the enduring legacy of Dolly the sheep.

Nature·2026
Same journal

Time to give hydration breaks the red card? What science says about keeping cool.

Nature·2026
Same journal

Universities are relying on AI-detection software to catch cheating. How well do the programs work?

Nature·2026
Same journal

Daily briefing: 'Cyborg' cockroaches breathe underwater with printed suit.

Nature·2026
See all related articles

The yeast SWI/SNF complex repositions nucleosomes along DNA through ATP-dependent sliding. This chromatin remodelling mechanism may be a general process for gene regulation.

Area of Science:

  • Molecular Biology
  • Gene Regulation
  • Chromatin Dynamics

Background:

  • Gene regulation relies on creating local chromatin structures permissive to transcription.
  • The yeast SWI/SNF complex is a key ATP-dependent remodeler of chromatin structure.
  • The precise mechanism of SWI/SNF-mediated chromatin disruption remains unclear.

Purpose of the Study:

  • To elucidate the pathway by which the yeast SWI/SNF complex remodels chromatin.
  • To investigate the mechanism of nucleosome repositioning by SWI/SNF.

Main Methods:

  • Utilized a model system to study yeast SWI/SNF complex activity.
  • Employed DNA barriers to probe nucleosome displacement mechanisms.
  • Analyzed ATP-dependent nucleosome repositioning.

Related Experiment Videos

Main Results:

  • Demonstrated that yeast SWI/SNF complex repositions nucleosomes in an ATP-dependent manner.
  • Showed that nucleosome repositioning favors attachment to acceptor sites on the same DNA molecule (in cis).
  • Found that DNA barriers impede SWI/SNF-mediated histone octamer displacement, indicating sliding or tracking.

Conclusions:

  • Concluded that SWI/SNF catalyzes nucleosome redistribution along DNA in cis.
  • Proposed that SWI/SNF-mediated nucleosome sliding is a general mechanism for ATP-dependent chromatin remodeling.
  • Highlighted the importance of SWI/SNF in establishing transcription-permissive chromatin topology.