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Updated: Mar 17, 2026

Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos

Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos

Published on: August 18, 2022

Genome-wide DNA methylation profiling in zebrafish.

P J Murphy¹, B R Cairns¹

¹University of Utah School of Medicine, Salt Lake City, UT, United States.

Methods in Cell Biology

|July 23, 2016

Summary

This summary is machine-generated.

Zebrafish offer a powerful model for studying DNA methylation, a key epigenetic mark regulating gene expression. This review details methods like MeDIP-Seq and BS-Seq for genome-wide DNA methylation analysis in zebrafish.

Keywords:

BS-Seq Bisulfite DNA methylation Epigenetics Genome-wide MeDIP-Seq RRBS-Seq Reprogramming

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Last Updated: Mar 17, 2026

Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos

Rapid and Efficient Spatiotemporal Monitoring of Normal and Aberrant Cytosine Methylation within Intact Zebrafish Embryos

Published on: August 18, 2022

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Published on: April 30, 2018

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Published on: June 29, 2018

Area of Science:

Epigenetics and Genomics
Developmental Biology
Comparative Genomics

Background:

Genomic DNA methylation is a crucial epigenetic mechanism that represses gene expression by influencing transcription factor binding and recruiting repressive chromatin modifiers.
This epigenetic mark plays a vital role in embryonic pluripotency, development, and tissue differentiation, exhibiting chemical stability, enzymatic reversibility, and germline heritability.
The conserved nature of DNA methylation machinery in zebrafish makes it a valuable model organism for studying mammalian epigenetic processes.

Purpose of the Study:

To review the utility of the zebrafish model for investigating DNA methylation dynamics.
To describe established methodologies for performing comprehensive, genome-wide DNA methylation analyses in zebrafish.

Main Methods:

Methylated DNA immunoprecipitation followed by sequencing (MeDIP-Seq)
Bisulfite sequencing (BS-Seq)
Reduced representation bisulfite sequencing (RRBS-Seq)

Main Results:

The study provides a comprehensive overview of established genome-wide DNA methylation analysis techniques applicable to zebrafish.
Detailed methods for MeDIP-Seq, BS-Seq, and RRBS-Seq are presented, enabling robust epigenetic profiling in this model organism.

Conclusions:

The zebrafish model, with its conserved DNA methylation machinery, is highly suitable for studying fundamental biological processes governed by epigenetic regulation.
The described methods facilitate in-depth genome-wide DNA methylation analysis, advancing research in epigenetics, development, and disease in a vertebrate model.