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Related Experiment Videos

Controlled DNA delivery systems.

D Luo1, K Woodrow-Mumford, N Belcheva

  • 1School of Chemical Engineering, Cornell University, Ithaca, New York 14850, USA.

Pharmaceutical Research
|September 1, 1999
PubMed
Summary
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Controlled release systems using biocompatible polymers effectively deliver DNA. These systems offer adjustable, low-cost options for gene therapy and DNA vaccination, maintaining DNA integrity.

Area of Science:

  • Biomaterials Science
  • Gene Delivery Technology
  • Polymer Chemistry

Background:

  • Gene-based pharmaceuticals require effective long-term delivery systems.
  • Current gene delivery methods are often inadequate for sustained therapeutic effects.
  • Biocompatible and biodegradable polymers present a promising alternative for controlled gene delivery.

Purpose of the Study:

  • To characterize controlled DNA delivery systems using implantable polymer matrices (EVAc) and injectable microspheres (PLGA, PLA).
  • To evaluate the encapsulation and release of various DNA sizes from these polymer systems.
  • To assess the integrity and functionality of DNA post-release.

Main Methods:

  • Encapsulation of herring sperm DNA and lambda DNA as model systems.

Related Experiment Videos

  • Fluoroassays for quantifying released DNA concentration.
  • Agarose electrophoresis to determine DNA size-dependent release rates.
  • Green Fluorescent Protein (GFP) gene used to confirm DNA integrity and functionality.

    • Main Results:

    • Successful encapsulation and release of small (0.1-0.6 kb, 1.9 kb) and large (48.5 kb) DNA molecules from EVAc, PLGA, and PLA systems.
    • DNA release from EVAc matrices was diffusion-controlled, with larger DNA molecules releasing more slowly.
    • Chemical and biological integrity of released DNA was preserved.
    • Release rate correlated with the DNA diffusion coefficient in water.

    Conclusions:

    • Developed controlled DNA delivery systems using FDA-approved, biocompatible/biodegradable polymers (EVAc, PLGA, PLA).
    • These systems are implantable and injectable, offering adjustable and low-cost DNA delivery.
    • Potential applications include in vivo gene delivery for DNA vaccination and gene therapy.