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Updated: Aug 15, 2026

Infinium Assay for Large-scale SNP Genotyping Applications
Published on: November 19, 2013
Chip-based genotyping by mass spectrometry
1Sequenom Inc., 11555 Sorrento Valley Road, San Diego, CA 92121, USA. ktang@sequenom.com
Abstract:
Silicon chips with immobilized target DNAs were used for accurate genotyping by mass spectrometry. Genomic DNAs were amplified with PCR, and the amplified products were covalently attached to chip wells via N-succinimidyl (4-iodoacetyl)aminobenzoate (SIAB) chemistry. Primer annealing, extension, and termination were performed on a 1-microl scale directly in the chip wells in parallel. Diagnostic products thus generated were detected in situ by using matrix-assisted laser desorption ionization mass spectrometry. This miniaturized method has the potential for accurate, high-throughput, low-cost identification of genetic variations.
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