Related Experiment Video

Updated: Aug 14, 2026

Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance
06:40

Quantitative Polymerase Chain Reaction (qPCR)-Based Rapid Diagnosis of Helicobacter pylori Infection and Antibiotic Resistance

Published on: July 28, 2023

Different penicillin-binding protein profiles in amoxicillin-resistant Helicobacter pylori

M P Dore1, D Y Graham, A R Sepulveda

  • 1Department of Medicine and Pathology, VA Medical Center and Baylor College of Medicine, Houston, TX 77030, USA.

Helicobacter
|September 1, 1999
PubMed
Abstract

Insights

Amoxicillin resistance in Helicobacter pylori may stem from alterations in penicillin-binding proteins (PBPs), specifically the absence of PBP D in resistant strains. This finding is crucial for understanding antibiotic resistance mechanisms.

Area of Science:

  • Microbiology
  • Antibiotic Resistance
  • Bacterial Physiology

Background:

  • Beta-lactam antibiotics inhibit bacterial peptidoglycan synthesis.
  • Amoxicillin-resistant Helicobacter pylori strains lacking beta-lactamase were identified.
  • Penicillin-binding proteins (PBPs) are targets for beta-lactams, and their alterations are linked to resistance.

Purpose of the Study:

  • To investigate the potential role of altered penicillin-binding proteins (PBPs) in amoxicillin resistance in Helicobacter pylori.
  • To compare PBPs between amoxicillin-sensitive and amoxicillin-resistant H. pylori strains.

Main Methods:

  • Five amoxicillin-resistant and three sensitive H. pylori strains were analyzed.
  • Penicillin-binding proteins (PBPs) were extracted and labeled in vivo with 3H-benzylpenicillin.
  • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and fluorography were used to analyze PBPs.

Main Results:

  • Four main PBPs were identified in amoxicillin-sensitive H. pylori strains.
  • Amoxicillin-resistant strains exhibited only three of the four main PBPs.
  • A specific PBP, designated PBP D (30-32 kD), was absent in resistant strains.

Conclusions:

  • The absence or alteration of PBP D is implicated in the amoxicillin-resistant phenotype of beta-lactamase-negative H. pylori.
  • PBP D is a potential key factor in amoxicillin resistance in H. pylori.
  • Further research into PBP D function is warranted to understand amoxicillin resistance.

More Related Videos

Gastric Mucosa Quantitative Polymerase Chain Reaction Analysis for Detecting Helicobacter pylori and Antibiotic Resistance05:23

Gastric Mucosa Quantitative Polymerase Chain Reaction Analysis for Detecting Helicobacter pylori and Antibiotic Resistance

Published on: March 7, 2025

Detection of Helicobacter pylori Infection and Antibiotic Resistance via Stool Quantitative Polymerase Chain Reaction Analysis04:56

Detection of Helicobacter pylori Infection and Antibiotic Resistance via Stool Quantitative Polymerase Chain Reaction Analysis

Published on: May 16, 2025

Related Concept Videos

Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy01:16

Treating Helicobacter pylori in Peptic Ulcers: Antimicrobial Therapy

Helicobacter pylori, a resilient gram-negative bacterium, can thrive in the stomach's harsh, acidic environment. Infection with H. pylori leads to a cascade of events within the stomach lining. One of the critical disruptions caused by this bacterium is the interference with somatostatin production, a hormone responsible for regulating acid secretion. This interference tips the balance, escalating acid secretion and diminishing bicarbonate levels. This imbalance compromises the defensive...
Factors Affecting Protein-Drug Binding: Drug-Related Factors01:18

Factors Affecting Protein-Drug Binding: Drug-Related Factors

Drug binding to proteins is a complex phenomenon influenced by various drug-related factors, each playing a significant role in the interaction between drugs and proteins within the body.
One crucial factor in drug-protein binding is the drug's lipophilicity or its affinity for fat. More lipophilic drugs tend to have higher binding extents. For example, highly lipophilic drugs like cloxacillin exhibit substantial protein binding, with as much as 95% of the drug binding to proteins. In contrast,...
Development of Antibiotic Resistance01:30

Development of Antibiotic Resistance

Antibiotic resistance is a major public health concern that arises when bacteria evolve mechanisms to withstand the effects of antibiotic treatments. This resistance can be intrinsic, acquired through genetic mutations, or transferred between bacteria via horizontal gene transfer. The development of antibiotic resistance poses significant challenges in treating bacterial infections and necessitates ongoing research to develop new therapeutic strategies.Intrinsic resistance occurs when bacterial...
Mechanism of Antibiotic Resistance in MRSA01:25

Mechanism of Antibiotic Resistance in MRSA

Antibiotic resistance in bacteria arises when microorganisms evolve the ability to withstand drugs designed to kill them or inhibit their growth, rendering once-effective treatments useless. This phenomenon, driven by genetic change and selection under antibiotic exposure, poses a profound threat to modern medicine. Mechanisms include drug-inactivating enzymes (e.g., β-lactamases), efflux pumps that eject antibiotics, mutations altering antibiotic targets, decreased drug uptake, and acquisition...
Inhibitors of Gram-positive Cell Wall Synthesis01:23

Inhibitors of Gram-positive Cell Wall Synthesis

Bacterial cell walls are typically rigid structures composed mainly of peptidoglycan, a mesh-like polymer that provides mechanical strength and maintains cell shape. The synthesis of peptidoglycan is a crucial process in bacterial growth and serves as a primary target for many antibiotics.Mechanism of Action of Beta-Lactam AntibioticsBeta-lactam antibiotics, such as penicillin, inhibit peptidoglycan synthesis in actively growing cells. These antibiotics share a characteristic four-membered...
Clinical Significance of Antibiotic Resistance01:25

Clinical Significance of Antibiotic Resistance

Methicillin-resistant Staphylococcus aureus (MRSA) presents a critical public health threat, arising from its capacity to resist β-lactam antibiotics due to acquisition of the mecA gene within the staphylococcal cassette chromosome mec (SCCmec). This gene encodes penicillin-binding protein 2a (PBP2a), which impairs binding efficacy of methicillin and other β-lactams. MRSA has evolved into distinct clonal lineages impacting humans and animals alike, reinforcing its significance within the One...