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Expression pattern of the Tbr2 (Eomesodermin) gene during mouse and chick brain development
A Bulfone1, S Martinez, V Marigo
1Telethon Institute of Genetics and Medicine (TIGEM), San Raffaele Biomedical Science Park, Milan, Italy. bulfone@tigem.it
Mechanisms of Development
|September 3, 1999
Summary
Researchers identified Tbr1 and Tbr2 genes in chickens and mice, revealing conserved roles in embryonic brain development. Their expression patterns offer insights into early developmental processes in both species.
Area of Science:
- Developmental Biology
- Genetics
- Neuroscience
Background:
- T-box genes are crucial for development, characterized by a conserved T-domain.
- Understanding T-box gene function is key to deciphering developmental processes.
Purpose of the Study:
- To clone and characterize chicken Tbr1 and murine/chicken Tbr2 genes.
- To investigate the expression patterns of Tbr1 and Tbr2 during embryogenesis in mice and chicks.
- To identify conserved developmental roles of these genes.
Main Methods:
- Gene cloning techniques.
- Chromosomal mapping of murine Tbr2.
- Whole-mount in situ hybridization or similar expression analysis techniques.
Main Results:
- Cloning of chicken Tbr1 and murine/chicken Tbr2 (orthologs of Xenopus eomesodermin).
- Murine Tbr2 mapped to chromosome 9.
- Both Tbr1 and Tbr2 exhibit conserved expression in the telencephalic pallium of mice and chicks.
- Chick Tbr2 shows dynamic expression during gastrulation.
Conclusions:
- Tbr1 and Tbr2 play conserved roles in the developing telencephalic pallium across species.
- Chick Tbr2 has a distinct role in early gastrulation.
- These findings contribute to understanding T-box gene function in vertebrate development.