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Cig30 and Pitx3 genes are arranged in a partially overlapping tail-to-tail array resulting in complementary
1The Wenner-Gren Institute, Arrhenius Laboratories F3, Stockholm University, S-106 91 Stockholm, Sweden.
The Journal of Biological Chemistry
|September 3, 1999
Summary
The mouse Cig30 gene, involved in brown adipose tissue recruitment, is located on chromosome 19. It is tightly linked to the Pitx3 homeobox gene, suggesting bidirectional transcription.
Area of Science:
- Genomics
- Molecular Biology
- Mouse Genetics
Background:
- The mouse Cig30 gene encodes a 30-kDa membrane glycoprotein.
- Cig30 is implicated in the recruitment of brown adipose tissue.
- Understanding the Cig30 gene structure and location is crucial for further research.
Purpose of the Study:
- To elucidate the genomic structure of the mouse Cig30 gene.
- To determine the chromosomal location of the Cig30 locus.
- To identify any genes located in proximity to Cig30.
Main Methods:
- Isolation of a lambda phage genomic DNA clone containing the entire mouse Cig30 gene.
- Fluorescence in situ hybridization (FISH) assay and interspecific backcross panel mapping.
- DNA sequencing of the isolated genomic clone.
Main Results:
- The Cig30 gene comprises four exons spanning 4 kilobase pairs.
- The Cig30 locus was mapped to the distal region of mouse chromosome 19, between the Tlx1 and Ins1 loci.
- A novel homeobox gene, Pitx3, was identified in the opposite orientation, tightly linked to Cig30.
Conclusions:
- The mouse Cig30 gene structure and localization were elucidated.
- The tight linkage and complementary transcript 3' ends of Cig30 and Pitx3 suggest bidirectional transcription.
- This finding provides evidence for a novel regulatory mechanism involving tightly linked genes.