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The 9-fluorenylmethoxycarbonyl (Fmoc) group and its use in oligonucleotide synthesis
H Schirmeister-Tichy1, G G Alvarado, W Pfleiderer
1Fakultät für Chemie, Universität Konstanz.
Nucleosides & Nucleotides
|September 4, 1999
Abstract:
The introduction of the base-labile 9-fluorenylmethoxycarbonyl (Fmoc) group into the exocyclic amino function of 2'-deoxynucleosides and their dimethoxytritylation and phosphitylation is described. The resulting key intermediates were investigated in the built-up of different oligodeoxyribonucleoside phosphate and thiophosphate chains which were deprotected under mild basic conditions leading to crude oligomers of high purity.