Related Experiment Videos
New techniques for the rapid characterization of oligonucleotides by mass spectrometry
J A McCloskey1, A B Whitehill, J Rozenski
1Department of Medicinal Chemistry, University of Utah, Salt Lake City 84112-5820, USA.
Nucleosides & Nucleotides
|September 4, 1999
Abstract:
Recent advances in combined HPLC/electrospray ionization-mass spectrometry provide effective new capabilities for the rapid characterization of oligonucleotides. Accurate mass measurements with errors < 0.3 Da, and determination of base and sugar modification and of nearest neighbor identities, can be routinely carried out on 10-100 component mixtures of RNA or DNA. These procedures are widely applicable in structural and analytical studies involving mixtures of oligonucleotides.