Related Experiment Video
Updated: Aug 13, 2026

Deep Proteome Profiling by Isobaric Labeling, Extensive Liquid Chromatography, Mass Spectrometry, and Software-assisted Quantification
Published on: November 15, 2017
New polymer-based prepacked column for the reversed-phase liquid chromatographic separation of peptides over the pH
J Mohammad1, B Jäderlund, H Lindblom
1Amersham Pharmacia Biotech AB, Uppsala, Sweden. jamil.mohammad@eu.apbiotech.com
Abstract:
The aim of this paper was to investigate the properties of a new column, Source 5RPC, for the separation of peptides at pH 2, 4.5, 7, 9 and 12 and to compare this product with similar polymer-based products available on the market. All columns were prepacked with 5 microm polystyrene-divinylbenzene polymer bead matrices and had dimensions of 150x4.6 mm I.D. Separations of angiotensin peptides were achieved on these columns using different equilibration solvents in the pH range 2-12 and elution with acetonitrile gradients. The separation of the peptide mixture obtained on Source 5RPC column was compared with that of two other commercially available polymer-based matrices. Method scouting and optimisation were carried out using the novel chromatography system, AKTA purifier.
Related Concept Videos
Principles Of Column Chromatography
Types Of Column Chromatography
Gel Filtration Chromatography
When the...
Gas Chromatography: Types of Columns and Stationary Phases
For an analyte to remain on the column for a sufficient amount of time, it must exhibit some level of compatibility (or...
High-Performance Liquid Chromatography: Introduction
In HPLC, two phases play a critical role in the separation process:
High-Performance Liquid Chromatography: Elution Process
Ion-Exchange Chromatography

