Empirical relationship between chiral selectivity and mobile phase modifier properties

Area of Science:

• Analytical Chemistry
• Chromatography
• Separation Science

Background:

• Chiral separations are crucial in pharmaceuticals and chemical industries.
• Predicting chiral selectivity is challenging due to complex analyte-selector interactions.
• Mobile phase composition significantly influences chromatographic performance.

Purpose of the Study:

• To establish an empirical relationship between mobile phase modifier properties and chiral selectivity.
• To develop a predictive model for chiral separations using different stationary and mobile phases.
• To assess the model's applicability with novel modifiers and analytes.

Main Methods:

• Utilized carbon dioxide and heptane-based mobile phases.
• Employed Pirkle-type and cellulosic stationary phases.
• Derived a two-parameter empirical equation to model mobile phase modifier effects.

Main Results:

• An accurate two-parameter model was developed for Pirkle-type phases with CO2 and heptane-based mobile phases.
• Similar predictive success was achieved for cellulosic phases using CO2-based mobile phases.
• Modeling was unsuccessful for heptane-based mobile phases with cellulosic stationary phases.

Conclusions:

• The derived empirical relationship effectively models chiral selectivity factors.
• The predictive model shows promise for optimizing chiral separations, particularly with CO2 mobile phases.
• Further investigation is needed for heptane-based mobile phases with cellulosic stationary phases.