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Related Experiment Videos

High throughput fluorescent CE-SSCP SNP genotyping.

D Gonen1, J Veenstra-VanderWeele, Z Yang

  • 1Laboratory for Developmental Neuroscience, Child and Adolescent Psychiatry, Department of Psychiatry, University of Chicago, MC3077, 5841 S Maryland Avenue, Chicago, Illinois 60637, USA. ed@yoda.bsd.uchi cago.edu

Molecular Psychiatry
|September 14, 1999
PubMed
Summary
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This study introduces capillary electrophoresis single-strand conformation polymorphism (CE-SSCP) for genotyping single nucleotide polymorphisms (SNPs). This method enables rapid, cost-effective SNP genotyping for genetic mapping and population studies.

Area of Science:

  • Genetics
  • Molecular Biology
  • Bioinformatics

Background:

  • Large-scale identification of single nucleotide polymorphisms (SNPs) is crucial for developing dense genetic maps.
  • Single-strand conformation polymorphism (SSCP) analysis is a common method for detecting DNA sequence variations in PCR products.
  • SNP genotyping in large populations is essential for genetic studies.

Purpose of the Study:

  • To develop a panel of 15 functional candidate SNPs using a novel genotyping method.
  • To assess the feasibility of using automated capillary electrophoresis SSCP (CE-SSCP) for high-throughput SNP genotyping.

Main Methods:

  • Utilized polymerase chain reaction (PCR) with fluorescent labeling.
  • Employed automated capillary electrophoresis SSCP (CE-SSCP) for analyzing single-stranded DNA migration differences.

Related Experiment Videos

  • Developed a panel of 15 functional candidate SNPs.

    • Main Results:

    • Demonstrated the development of 15 functional candidate SNPs.
    • Showcased the feasibility of rapid and cost-effective CE-SSCP SNP genotyping using current technology.
    • Estimated a throughput of 135,000 genotypes per year with a single capillary instrument.

    Conclusions:

    • CE-SSCP is a viable method for genotyping SNPs in large sample populations.
    • Automated CE-SSCP offers a rapid and low-cost approach for SNP genotyping.
    • Potential for increased throughput with parallel multiple array capillary electrophoresis.