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Related Experiment Videos

Lactate dehydrogenase isoenzyme patterns in cetaceans.

T H Reidarson1, J McBain, L M Dalton

  • 1Sea World of California, San Diego 92109, USA.

Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians
|September 14, 1999
PubMed
Summary

Serum lactate dehydrogenase (LDH) isoenzyme analysis in cetaceans revealed distinct profiles differentiating i.m. injections and azole therapy. These patterns aid in diagnosing enzyme elevations when other methods are limited.

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Area of Science:

  • Veterinary Medicine
  • Biochemistry
  • Marine Mammal Science

Background:

  • Serum lactate dehydrogenase (LDH) isoenzyme analysis is a valuable diagnostic tool.
  • Understanding LDH isoenzyme profiles in cetaceans is crucial for accurate health assessments.
  • Distinguishing pathological from non-pathological enzyme elevations can be challenging in marine mammals.

Purpose of the Study:

  • To analyze serum lactate dehydrogenase (LDH) isoenzyme activity in cetaceans.
  • To identify distinct LDH isoenzyme profiles associated with specific treatments or conditions.
  • To evaluate the utility of LDH isoenzyme patterns in differentiating causes of elevated serum enzyme levels in cetaceans.

Main Methods:

  • Analysis of serum lactate dehydrogenase (LDH) isoenzyme activity in cetacean blood samples.

Related Experiment Videos

  • Comparison of LDH isoenzyme profiles from animals undergoing i.m. injection and azole therapy.
  • Assessment of LDH isoenzyme patterns in clinically normal animals with elevated enzyme levels.
  • Evaluation of the impact of hemolysis and sample handling (refrigeration, freezing) on LDH isoenzyme patterns.

    • Main Results:

    • Distinct LDH isoenzyme profiles were observed in cetaceans treated with i.m. injections and those receiving azole therapy.
    • A unique LDH isoenzyme pattern was occasionally found in clinically normal animals with elevated total transaminase and LDH activity.
    • Mild to moderate hemolysis, refrigeration, or freezing did not significantly alter LDH isoenzyme patterns.
    • Severe hemolysis caused artifactual changes in LDH isoenzyme profiles, mimicking injection-related patterns but to a lesser extent.

    Conclusions:

    • Serum LDH isoenzyme activity patterns can provide valuable corroborative evidence in cetacean diagnostics, especially when other methods are limited.
    • LDH isoenzyme analysis is particularly useful for differentiating non-specific enzyme elevations from non-pathological elevations caused by i.m. injections or azole therapy.
    • These findings enhance the diagnostic capabilities for assessing enzyme abnormalities in marine mammals.