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Chorionic villus sampling prior to pregnancy termination, a tool for forensic paternity testing
A Reshef1, P Brauner, M Shpitzen
1Forensic Biology Laboratory, Israel Police National Headquarters, Jerusalem, Israel.
Journal of Forensic Sciences
|September 16, 1999
Summary
Chorionic villus sampling (CVS) offers a clean fetal DNA source for forensic paternity testing before pregnancy termination. Confined placental mosaicism (CPM) may affect DNA analysis results.
Area of Science:
- Reproductive biology
- Forensic genetics
- Molecular diagnostics
Background:
- Chorionic villus sampling (CVS) is a prenatal diagnostic technique.
- Paternity testing traditionally uses post-mortem or abortion-derived tissues.
- These tissues can be contaminated with maternal DNA.
Observation:
- Pre-termination CVS provides extra-embryonic membranes.
- These membranes offer an uncontaminated source of fetal DNA.
- Confined placental mosaicism (CPM) is a condition where placental cells have different genetic material than fetal cells.
Findings:
- CVS allows for fetal genotyping from uncontaminated placental tissue.
- Polymerase chain reaction (PCR)-based analyses of short tandem repeats (STRs) and D1S80 loci are used.
- The presence of CPM can impact the accuracy of these genetic analyses.
Implications:
- CVS is a viable method for forensic paternity testing.
- Understanding CPM is crucial for interpreting genetic results from CVS.
- This technique enhances the reliability of paternity determination in specific legal and medical contexts.