Properties of rat cone-mediated electroretinograms during light adaptation
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Updated: Oct 19, 2026

Simultaneous ex vivo Functional Testing of Two Retinas by in vivo Electroretinogram System
Published on: May 6, 2015
Y Goto1, S Tobimatsu, J Shigematsu
1Kyushu University Neurological Institute Department of Clinical Neurophysiology Fukuoka, Japan. ygoto@neurophy.med.kyushu-u.ac.jp
Purpose:
Our aim was to better understand how to isolate the cone-mediated response in rats. Therefore, we studied the difference of ERGs in the course of light adaptation between 2 and 20 Hz stimulus frequencies.
Methods:
A total of 90 rats divided into 18 different groups were used following overnight dark adaptation. ERGs were recorded against 3 different adapting field luminances (1.15, 1. 50 or 1.75 log cd/m(2)) with a combination of 3 stimulus flash intensities (0.86, 1.30 or 2.03 log cd sec/m( 2)). The responses were obtained at 2 minute intervals for 25 minutes of light adaptation.
Results:
The response of the rat cone ERG was large despite the small number of cones. The mean amplitude increased systematically from the dark-adapted value requiring more than 15 minutes to reach an asymptote at 2 Hz stimulation, but only 10 minutes at 20 Hz stimulation. The 2 Hz adaptation curves had biphasic pattern compared to the monophasic 20 Hz curve. This second increase in the amplitude at 2 Hz appeared at around 7-8 minutes as a function of adaptation time. This tendency was most evident when using a low adapting field luminance with a high flash intensity.
Conclusions:
Our results suggest that the rods intrude much more during light adaptation at 2 Hz stimulation in rodents than in humans. Therefore, 20 Hz flicker stimulation can better isolate more the cone-mediated function than 2 Hz stimulation during the course of light adaptation in rats. Furthermore, the functional characteristics of the cone in rats may be different from that in humans.
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