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[The expression of p16, CDK4 and pRb in hepatocellular carcinomas]
Objective:
To make an analysis of the expression of p16 gene, CDK4 and retinoblastoma protein(pRb) in human hepatocellular carcinoma(HCC).
Methods:
Application of immunohistochemistry to 40 cases of HCC and their adjacent liver tissues.
Results:
The abnormal expressions of p16, CDK4 and pRb were 94.44%(34/36), 40.00%(16/40) and 45.71%(16/35) respectively. Rb protein was strongly stained in 13/34(38.23%) cases lacking or weak staining with p16, which showed a negative relation between the expressions of Rb and p16. The abnormal expressions of both p16 and Rb were 15/34(44.11%) cases, indicating that except p16, there are some other ways to regulate Rb. In 14 cases of CDK4 overexpression, most of them (92.85%) presented p16 aberration, 50.00% of them were observed with pRb abnormal expression, 46.15% of them were along with the alterations of both p16 and pRb. There was no significant correlation between p16 or CDK4 or pRb abnormal expression and HCC malignant grade.
Conclusion:
The data show that the aberrant expression of p16, CDK4 and pRb may play a role in the carcinogenesis of HCC. The changes of p16 expression is prominent.