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Detection of nitrosyl-iron complexes by proton-electron-double-resonance imaging
A Mülsch1, D J Lurie, I Seimenis
1Institut für Kardiovaskuläre Physiologie, Klinikum der Johann Wolfgang Goethe-Universität, Frankfurt, Germany. muelsch@em.uni-frankfurt.de
Free Radical Biology & Medicine
|September 18, 1999
Summary
Nitrosyl-iron complexes (NICs) can be detected using proton-electron-double-resonance imaging (PEDRI). This technique shows potential for noninvasive in vivo imaging of the nitrogen monoxide radical (NO*) in biological tissues.
Area of Science:
- Biophysics
- Medical Imaging
- Biochemistry
Background:
- Nitrogen monoxide radical (NO*) forms paramagnetic mono- and dinitrosyl-iron complexes (NICs) in biological tissues.
- These complexes possess unpaired electrons capable of dynamic nuclear polarization with water protons.
- This property is crucial for enhancing magnetic resonance (MR) imaging signals.
Purpose of the Study:
- To evaluate the suitability of NICs as contrast agents for in vivo NO* imaging.
- To explore the application of proton-electron-double-resonance imaging (PEDRI) for detecting NICs.
- To establish a noninvasive imaging technique for NO* in biological systems.
Main Methods:
- Utilized phantom solutions of synthetic nitrosyl-iron complexes (NICs) to assess PEDRI signal alteration.
- Compared the signal alteration induced by dinitrosyl-iron complex (DNIC) with serum albumin versus mononitrosyl-iron complex (MNIC) with dithiocarbamate.
- Exposed rat liver to sodium nitroprusside (SNP) for ex vivo and in situ perfusion, analyzing electron spin resonance (ESR) spectra and cross-sectional PEDRI images.
Main Results:
- Synthetic NICs altered PEDRI image signal intensity.
- DNIC with serum albumin showed a significantly larger signal alteration than MNIC with dithiocarbamate.
- SNP-exposed rat livers exhibited characteristic MNIC and DNIC ESR spectra, with MNIC signal decreasing and DNIC signal increasing upon storage.
- PEDRI images of SNP-exposed livers showed an initial weak signal that increased over time.
Conclusions:
- Nitrosyl-iron complexes (NICs) are detectable using proton-electron-double-resonance imaging (PEDRI).
- NICs demonstrate potential as contrast agents for noninvasive in vivo imaging of the nitrogen monoxide radical (NO*).
- PEDRI offers a promising avenue for future NO* imaging applications in biological research and diagnostics.