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Related Experiment Videos

Detecting APC-resistant factor V: a functional method without plasma dilution.

D B Rylatt1, C Hohnen-Behrens, R L Pilgrim

  • 1Gradipore Research Laboratories, Riverside Corporate Park, North Ryde, New South Wales, Australia.

Blood Coagulation & Fibrinolysis : an International Journal in Haemostasis and Thrombosis
|September 24, 1999
PubMed
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A novel method detects activated protein C (APC)-resistant factor V, including factor V Leiden, using snake venom reagents. This test effectively distinguishes carriers across various patient groups without needing deficient plasma.

Area of Science:

  • Hematology
  • Clinical Diagnostics
  • Toxicology

Background:

  • Activated protein C (APC) resistance is a significant risk factor for thrombosis.
  • Factor V Leiden is the most common inherited cause of APC resistance.
  • Accurate detection of APC resistance is crucial for patient management, especially in those on anticoagulant therapy.

Purpose of the Study:

  • To develop and validate a novel diagnostic method for detecting APC-resistant factor V, including factor V Leiden.
  • To assess the utility of the method in diverse patient populations, including those on oral anticoagulants, heparin, or with lupus anticoagulant.
  • To evaluate the method's performance without the need for factor-V-deficient plasma.

Main Methods:

  • Incubation of patient plasma with dilute Southern Copperhead (Agkistrodon contortrix contortrix) venom to activate endogenous protein C.

Related Experiment Videos

  • Performance of a dilute Russell's viper venom time (dRVVT) test following venom incubation.
  • Comparison of dRVVT results between individuals with and without APC resistance in various clinical groups.

    • Main Results:

    • A marginal prolongation of dRVVT was observed in individuals with APC-resistant factor V (1.14 +/- 0.14 s).
    • Non-carriers showed significantly longer dRVVT (2.38–2.69 s) across normal donors and patient groups.
    • The method successfully discriminated between carriers and non-carriers in all tested patient cohorts.

    Conclusions:

    • The described snake venom-based method provides a reliable and accessible means for detecting APC-resistant factor V and factor V Leiden.
    • This diagnostic approach is suitable for patients undergoing various anticoagulant therapies and those with lupus anticoagulant.
    • The findings suggest a potential subgroup of oral anticoagulant patients with moderate dRVVT prolongation and low functional protein C, indicating elevated thrombotic risk.