[Characteristics of lipopolysaccharide from Pseudomonas fluorescens (biovar I)]

Lipopolysaccharide (LPS) of the Pseudomonas fluorescens strain IMV 7769 (biovar I) was isolated and investigated. Fractions of the structural parts of the LPS macromolecule, lipid A, the core oligosaccharide, and the O-specific polysaccharide (O-PS), were obtained in a homogeneous state. 2-Hydroxydecanoic, 3-hydroxydecanoic, dodecanoic, 2-hydroxydodecanoic, 3-hydroxydodecanoic, hexadecanoic, octadecanoic, hexadecenoic, and octadecenoic fatty acids were identified in lipid A. In the hydrophilic moiety of lipid A, after acid hydrolysis, several amino acids, phosphoethanolamine, glucosamine, and three unidentified peaks forming a separate cluster together with glucosamine were found. Lipid A was shown to be phosphorylated. Glucose, fucose, rhamnose, glucosamine, galactosamine, two unidentified amino sugars, 2-keto-3-deoxyoctulonic acid (KDO), heptose, ethanolamine, phosphoethanolamine, and alanine were identified in the core oligosaccharide. O-PS of the LPS consisted of repeating trisaccharide fragments that included residues of amino sugars: 4-acetamido-4,6-dideoxy-D-galactose, 2-acetamido-2,6-dideoxy-D-glucose, and 2-acetamido-2,6-dideoxy-L-glucose. During growth, the strain under study excreted exocellular LPS (ELPS) into the medium. The LPS studied was similar to the LPS of the earlier investigated strains P. fluorescens (biovar I) IMV 1152 and IMV 1433 in the structure of O-PS, but differed from them in the composition of both lipid A and the core oligosaccharide. The LPS of the strain studied differed from LPS of the type strain P. fluorescens IMV 4125 (ATCC 13525) in all characteristics determined.