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[Association of MAP kinases with the endoplasmic reticulum in NIH3T3 (HER14) and A431 cells]
A M Arnautov1, I P Arnautova, N N Zheleznova
1Institute of Cytology, Russian Academy of Sciences, St. Petersburg.
Tsitologiia
|September 25, 1999
Abstract:
Intracellular distribution of p42/p44 MAP-kinases in HER14 and A431 cell lines was investigated. Using subcellular fractionation and immunofluorescence approaches we have shown that in quiescent cells of both types MAP-kinases are associated with endoplasmic reticulum. Moreover, ER-localized MAP-kinases were shown to exist only in a nonphosphorylated form. In HER14 cells the epidermal growth factor (EGF) elevates the level of the ER-associated MAP-kinases. In contrast, exposure of A431 cells to EGF leads to a significant decrease in the observed association. The physiological role of this association is discussed.