Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reprogramming of gene expression during preimplantation development.

R M Schultz1, W Davis, P Stein

  • 1Department of Biology, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6018, USA. rschultz@sas.upenn.edu

The Journal of Experimental Zoology
|September 25, 1999
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Streamlining Global Germplasm Exchange: Integrating Scientific Rigor and Common Sense to Exclude Phantom Agents from Regulation.

Plant disease·2025
Same author

The role of thoracic surgery in the management of complicated acute and post-acute COVID-19 pneumonia (PASC).

Rozhledy v chirurgii : mesicnik Ceskoslovenske chirurgicke spolecnosti·2024
Same author

[Postoperative imaging of the shoulder].

Radiologie (Heidelberg, Germany)·2022
Same author

Regenerative abilities of a nanofiber wound dressing based on polylactide.

Rozhledy v chirurgii : mesicnik Ceskoslovenske chirurgicke spolecnosti·2021
Same author

Development of a new peptide-bead coupling method for an all peptide-based Luminex multiplexing assay for detection of Plasmodium falciparum antibody responses.

Journal of immunological methods·2021
Same author

Negative pressure wound therapy updates for 2021.

Rozhledy v chirurgii : mesicnik Ceskoslovenske chirurgicke spolecnosti·2021

Early mammalian development involves reprogramming gene expression. DNA replication and histone deacetylase activity regulate this transition, impacting gene expression crucial for early embryonic development.

Area of Science:

  • Developmental Biology
  • Epigenetics
  • Gene Regulation

Background:

  • Early mammalian development requires the oocyte to transition into totipotent blastomeres.
  • This transformation involves significant reprogramming of gene expression patterns.
  • The maternal-to-zygotic transition, where the embryo becomes transcriptionally active, is key.

Purpose of the Study:

  • To investigate the role of DNA replication in early gene expression.
  • To explore the mechanisms underlying the development of transcriptional repression in the 2-cell stage.
  • To determine the involvement of chromatin structure and histone deacetylases in this process.

Main Methods:

  • Studying gene expression (eIF-1A, TRC) at different embryonic stages.
  • Inhibiting DNA replication rounds.

Related Experiment Videos

  • Treating embryos with histone deacetylase inhibitors.

    • Main Results:

    • The first round of DNA replication is essential for eIF-1A and TRC gene expression and global transcription.
    • Inhibiting the second round of DNA replication prevents the decrease in eIF-1A, TRC, and global gene expression.
    • Histone deacetylase inhibition prevents the decrease in eIF-1A and TRC expression and inhibits the development of a transcriptionally repressive state.

    Conclusions:

    • DNA replication plays a critical role in regulating gene expression during early mammalian development.
    • Histone deacetylase activity is involved in establishing a transcriptionally repressive state during the 2-cell stage.
    • Chromatin remodeling, specifically histone deacetylation, provides a mechanism for repressing gene expression in early embryos.