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Updated: Oct 19, 2026
Expression and Purification of Nuclease-Free Oxygen Scavenger Protocatechuate 3,4-Dioxygenase
Published on: November 8, 2019
Purification and characterization of a novel naphthalene dioxygenase from Rhodococcus sp. strain NCIMB12038
M J Larkin1, C C Allen, L A Kulakov
1The Questor Centre, The Queen's University of Belfast, Belfast BT9 5AG, Northern Ireland. M.Larkin@qub.ac.uk
Abstract:
We report here the characterization of the catalytic component (ISP(NAR)) of a new naphthalene dioxygenase from Rhodococcus sp. strain NCIMB12038. The genes encoding the two subunits of ISP(NAR) are not homologous to their previously characterized counterparts in Pseudomonas. The deduced amino acid sequences have only 33 and 29% identity with the corresponding subunits in Pseudomonas putida NCIB 9816-4, for which the tertiary structure has been reported.
