Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Topics in prion cell biology.

J P Brockes1

  • 1Department of Biochemistry and Molecular Biology University College London Gower Street, London, WC1E 6BT, UK. j.brockes@ucl.ac.uk

Current Opinion in Neurobiology
|October 6, 1999
PubMed
Summary

Prion protein (PrP) research reveals its role in neuropathogenesis and normal function through copper interactions. Yeast prion studies explore aggregated structures and cellular regulation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Regeneration as an evolutionary variable.

Journal of anatomy·2001
Same author

Mammalian postmitotic nuclei reenter the cell cycle after serum stimulation in newt/mouse hybrid myotubes.

Current biology : CB·2001
Same author

The expression pattern of tomoregulin-1 in urodele limb regeneration and mouse limb development.

Mechanisms of development·2001
Same author

Generation of mononucleate cells from post-mitotic myotubes proceeds in the absence of cell cycle progression.

Differentiation; research in biological diversity·2001
Same author

Plasticity of retrovirus-labelled myotubes in the newt limb regeneration blastema.

Developmental biology·2000
Same author

Thrombin regulates S-phase re-entry by cultured newt myotubes.

Current biology : CB·1999

Area of Science:

  • Neuroscience
  • Biochemistry
  • Cell Biology

Background:

  • Transmembrane prion protein (PrP) is implicated in neuropathogenesis.
  • PrP's interaction with copper ions is crucial for understanding its normal function.
  • Yeast prions offer models for studying prion structure and cellular dynamics.

Purpose of the Study:

  • Investigate the role of transmembrane PrP in neuropathogenesis.
  • Analyze factors regulating PrP appearance at the endoplasmic reticulum.
  • Explore the structural and functional aspects of yeast prions.

Main Methods:

  • Analysis of transmembrane PrP and associated endoplasmic reticulum factors.
  • Study of PrP mutations influencing its cellular localization.
  • Investigation of copper ion interactions with PrP.
  • Structural and phenotypic analysis of yeast prion conformers.

Main Results:

  • Identified importance of transmembrane PrP in specific neuropathogenic contexts.
  • Characterized transacting factors and PrP mutations affecting its ER presence.
  • Highlighted copper ion interactions as key to PrP's normal function.
  • Detailed structure, phenotype, and regulation of yeast prion aggregates.

Conclusions:

  • Transmembrane PrP plays a significant role in certain neuropathological conditions.
  • Copper-PrP interactions are vital for understanding normal PrP function.
  • Yeast prions provide valuable insights into prion aggregation and cellular control mechanisms.

Related Experiment Videos