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Solid-phase extraction method for patulin in apple juice and unfiltered apple juice
1U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, Washington, DC 20204, USA.
Journal of AOAC International
|October 8, 1999
Summary
A new solid-phase extraction method efficiently isolates patulin, a mycotoxin in apples. This method offers high recovery rates for accurate patulin analysis in apple juice.
Area of Science:
- Food Science
- Analytical Chemistry
- Mycotoxicology
Background:
- Patulin is a mycotoxin found in apples affected by mold.
- Regulatory limits for patulin in food products exist in many countries.
- Traditional patulin analysis often involves liquid-liquid extraction.
Purpose of the Study:
- To develop a solid-phase extraction (SPE) method for patulin in apple juice.
- To provide an efficient and accurate analytical method for patulin determination.
- To meet the need for improved patulin testing in food safety.
Main Methods:
- Developed an SPE method using a macroporous copolymer cartridge.
- Employed specific washing steps with sodium bicarbonate and acetic acid.
- Eluted patulin using acetonitrile in anhydrous ethyl ether.
- Quantified patulin using reversed-phase liquid chromatography with UV detection.
Main Results:
- Achieved high recoveries for patulin, ranging from 93% to 104%.
- Demonstrated method effectiveness for both apple juice and unfiltered apple juice.
- Validated the method for patulin concentrations between 20-100 micrograms/L.
Conclusions:
- The developed SPE method is effective for patulin extraction from apple juice.
- This method provides accurate and reliable quantification of patulin.
- Offers a viable alternative to traditional extraction techniques for food safety analysis.