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Related Experiment Videos

Sol-gel-based biosensor for use in stroke treatment.

S A Grant1, R S Glass

  • 1Center for Biomedical Engineering, Michigan Technological University, Houghton 49931, USA. sagrant@mtu.edu

IEEE Transactions on Bio-Medical Engineering
|October 8, 1999
PubMed
Summary

A novel fiber optic biosensor detects D dimer, a marker for blood clots. This technology shows promise for diagnosing stroke-related occlusions and guiding treatment.

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Area of Science:

  • Biomedical Engineering
  • Optical Sensing
  • Clinical Diagnostics

Background:

  • Soft blood clots dissolve, releasing fibrinolytic products.
  • D dimer antigens indicate the dissolution of cross-linked fibrin clots.
  • Elevated D dimer levels are a clinical indicator for stroke-related occlusions.

Purpose of the Study:

  • To develop a fiber optic biosensor for detecting D dimer antigens.
  • To create a selective sensor for fibrinolytic products associated with blood clot lysis.
  • To assess the sensor's performance in clinically relevant conditions.

Main Methods:

  • Immobilizing fluorescein-labeled D dimer antibodies on an optical fiber tip using silica sol-gel dip coating.
  • Measuring changes in fluorescence intensity upon D dimer antigen binding.
  • Testing sensor response in phosphate buffered saline, human plasma, and blood.
  • Evaluating sensor stability and antibody viability over time.

Main Results:

  • The biosensor demonstrated a decrease in fluorescence intensity correlated with D dimer antigen concentration.
  • Calibration plots were established in the clinically relevant range of 0.54 to 6 micrograms/ml.
  • Encapsulated antibodies remained viable for at least 4 weeks when stored at 4°C.
  • Sensor performance was evaluated in various biological matrices.

Conclusions:

  • A fiber optic biosensor for D dimer detection has been successfully developed.
  • The sensor shows potential for diagnosing stroke due to vascular occlusions.
  • Further development aims to integrate this sensor with catheter-based microtools for stroke treatment.

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